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KMID : 1094720100150010191
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 1 p.191 ~ p.198
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Protective Effect of Amphiroa dilatata on ROS Induced Oxidative Damage and MMP Expressions in HT1080 Cells
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Sher Bahadar Khan
Kong Chang-Suk
Kim Jung-Ae
Kim Se-Kwon
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Abstract
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Antioxidative and matrix metalloproteinase (MMP) inhibitory effects of methanolic extract from Amphiroa dilatata were investigated in HT1080 cells. Treatment with A. dilatata extract suppressed the intensity of electron spin resonance signal on the generation of DPPH radicals in a cell-free system and intracellular reactive oxygen species (ROS) formation by 2’,7’-dichlorofluorescin diacetate (DCFH-DA). Radical simulated oxidation of membrane proteins and lipids were also inhibited by treatment with A. dilatata extract in a concentration-dependent manner. In addition, a protective effect on oxidative damage of purified genomic DNA was observed in the presence of A. dilatata extract. These results revealed that A. dilatata has an excellent scavenging ability on ROS-induced oxidative damage. Moreover, this extract reduced the expression levels of MMP-2 and -9 in gelatin zymography, and RT-PCR analysis without any cytotoxic influence. Therefore, A. dilatata has a promising potential as a valuable source to be used as ingredient in functional foods, related to oxidative damage.
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KEYWORD
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Amphiroa dilatata, MMP-2, MMP-9, reactive oxygen species
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